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Williams GR Role of Thyroid Hormones in Skeletal Development and Bone Maintenance

Blood glucose was determined using a handheld glucometer (OneTouch Ultra Easy, LifeScan) following an overnight fast, and plasma insulin levels were quantified by ELISA according to the manufacturers protocol (Abcam)

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Copper deficiency can result from impaired absorption (due to alkaline pH, use of antiacid drugs, or food complexes such as phytic acid or other minerals), conditions promoting copper loss (e.g., nephrosis), or total parenteral nutrition

This mechanism is particularly relevant for Indian skin tones, where UV exposure and pollution significantly accelerate pigmentation concerns